Electron Microscopic Autoradiography in Inner Ear Pathological Study
- VernacularTitle:电镜放射自显影技术在内耳病理研究中的应用
- Author:
Guohui NIE
;
Jibao WANG
- Publication Type:Journal Article
- Keywords:
Electron microscopy Autoradiography Inner ear Pathology
- From:
Journal of Audiology and Speech Pathology
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the technique of electron microscopic autoradiography in inner ear pathology.Methods The thin sections that was labeled with the thymidine-H3(3H-TdR)were prepared.The labeled specimen were processed for electron microscopic autoradiography(EM-ARG)after treatment with emulsions,development and fixation.Results Incorporation of 3H-TdR was seen over regenerated cells in the experimental papilla in regions of hair cell loss.Conclusion This methocl gives a good localization of the label at the ultrastructural level.
