Effects of FAK phosphorylation on adhesion and migration of smooth muscle cells stimulated by fibronectin
- VernacularTitle:粘着斑激酶活化对平滑肌细胞粘附和迁移的影响
- Author:
Hang YIN
;
Lihui WANG
;
Xu PENG
;
Yong HUO
;
Chunfang XIA
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Oligonucleotides, antisense;
Muscle, smooth, vascular;
Cells;
Adhesions
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of focal adhesion kinase (FAK) phosphorylation on the adhesion and migration of smooth muscle cells (SMCs) stimulated by fibronectin (FN). METHODS: Cultured SMCs were stimulated by different concentrations of FN.FAK expression and phosphorylation were detected by immunoprecipitation and Western blot. To investigate the modulating effect of FAK on tyrosine phosphorylation and SMCs adhesion and migration, FAK antisense oligodeoxynucleotides (ODNs) were transfected into SMCs by actionic lipid method. RESULTS: FAK expression increased when SMCs adhesion and migration were induced by FN at different concentrations used in the experiment. However, FAK phosphorylation was only observed by FN stimulation at concentration of 20 mg/L. FAK antisense ODNs inhibited FAK phosphorylation magnificently. The migration rates of SMCs were reduced by 17.89%-27.67% when FN was used at concentration from 5 mg/L to 60 mg/L. The decreased migrating cell numbers were showed the same patterns. The apoptotic SMCs were 33.57% higher than that control ( P
