The expression of the mouse ?_2 microglobulin in NIH3T3 cells transfected with antisense RNA
- VernacularTitle:小鼠?_2m正反义核酸表达载体转染NIH3T3细胞后的表达
- Author:
Ying MENG
;
Shaoliang HUANG
;
Shunon LI
- Publication Type:Journal Article
- Keywords:
Genes;
Mice;
Genes, MHC classⅠ;
? 2 microglobulin;
RNA, antisense;
Cells;
Transfection
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The expression of the mouse ? 2 microglobulin (? 2 m) in NIH3T3 cells transfected with the mouse ? 2m sense and antisense RNA was detected to clarify the effect of mouse ? 2m sense and antisense RNA on the expression of MHC classⅠgene. METHODS: The mouse sense and antisense RNA, pcDNA3-? 2mSN and pcDNA3-? 2mAN, were constructed and were transfected into NIH3T3 cells by lipofectamine. RT-PCR and Western blot were used to detect the expression of ? 2m in those cells. RESULTS: The expression of the mouse ? 2m in the cells transfected with pcDNA3-? 2mSN was increased, while it was decreased in those cells transfected with pcDNA3-? 2mAN. CONCLUSION: pcDNA3 -? 2mAN can downregulate the expression of the ? 2m in NIH3T3 cells.
