Oxysterols downregulate tissue inhibitor of metalloproteinase-1expression in vascular smooth muscle cells
- VernacularTitle:氧化型胆固醇下调血管平滑肌细胞金属蛋白酶组织抑制剂基因表达
- Author:
Ming CUI
;
Fengrong CHEN
;
Qinghua SONG
;
Yingba ZHU
- Publication Type:Journal Article
- Keywords:
Cholesterol;
Muscle,smooth,vascular;
Metalloproteinases;
\ Gene expression;
\ Rabbits
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of oxysterols on matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in vascular smooth muscle cells. METHODS: Rabbit aortic vascular smooth muscle cells were cultured in vitro and incubated with cholesterol, Triol and 25-hydroxycholesterol (25-OH), respectively. Slot blot was used to detect the mRNA expression level of TIMP-1 in vascular smooth muscle cells (VSMCs); meanwhile the protein expression level of MMP-9 and TIMP-1 was detected by immunohistology. RESULTS: Triol and 25-OH inhibited the expression of TIMP-1 compared with control and cholesterol, but have no effect on expression of MMP-9. CONCLUSION: Both Triol and 25-OH downregulated TIMP-1 expression in VSMCs .
