Role of insulin in the regulation of transforming growth factor-?and its receptors on vascular smooth muscle cells from spontaneously hypertensive rats
- VernacularTitle:胰岛素对自发型高血压大鼠血管平滑肌细胞TGF?及其受体的调节作用(英文)
- Author:
Huili LI
;
Dingjiu HUANG
- Publication Type:Journal Article
- Keywords:
Insulin;
Hypertension;
Rats;
Receptors, transforming growth factor beta;
Muscle, smooth, vascular
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of insulin on the proliferation and expression of transforming growth factor ? 1 (TGF ? 1) and its receptors in aortic vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) and from the normotensive Wistar-Kyoto(WKY) rats. METHODS: VSMCs from aortas of WKY rats and SHR were prepared by an explant method. The effect of insulin on VSMC proliferation were measured by cell counting and flow cytometry analysis, respectively. Quantitative reverse transcription polymerase chain reaction (RT-PCR) technique was used to probe TGF ? 1 and its receptor mRNA on VSMC. RESULTS: Insulin enhanced the proliferation of VSMC from SHR in a dose-dependent manner ( P 0 05). CONCLUSIONS: The responses to insulin of TGF ? 1 and its receptor in SHR and WKY are significantly different. This abnormal regulation by insulin may be associated with an exaggerated proliferative response in SHR derived cells.
