Factors involved in regulation of polymorphonuclear neutrophils apoptosis in vitro
- VernacularTitle:调控中性白细胞凋亡有关因素的体外实验
- Author:
Ren LIU
;
Kunlun TIAN
;
Nan XIAO
;
Youfang DIAO
- Publication Type:Journal Article
- Keywords:
Neutrophils;
Apoptosis;
Tumor necrosis factor;
Interleukin-6
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: This study aimed at elucidated the possibility that prevent tissue from secondary injury by regulating polymorphonuclear neutrophil (PMN) apoptosis in vitro . METHODS:Neutrophils, isolated from peripheral blood, were incubated with sodium arsenite (Ars), tumor necrosis factor (TNF-?), interleukin-6 (IL-6), burned serum and traumatic serum, respectively. Apoptosis rate, expression of CD11b, respiratory burst and concentration of Ca 2+ were then measured. RESULTS:The elevation of PMN apoptosis rate was Ars concentration dependent, but activated PMN became insensitive to Ars. IL-6 delayed PMN apoptosis (compared with control at 24 h, P
