Analysis of the distribution and clonality of TCR V?T cells in cord blood
- VernacularTitle:脐血中TCRV?亚家族T细胞的分布和克隆性分析
- Author:
Shaohua CHEN
;
Yangqiu LI
;
Lijian YANG
;
Yuping ZHANG
;
Qin LI
- Publication Type:Journal Article
- Keywords:
Fetal blood;
Receptor, antigen, T cell;
Genes
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the distribution and clonality of TCR V? subfamily T cells in cord blood. METHODS: The CDR3 of TCR V? 24 subfamily genes were amplified in mononuclear cells from 13 cases of cord blood. To observe the usage of TCR V? repertoire, the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluate clonality of the detectable TCR V? T cells. Peripheral bloods from 10 cases of normal individuals and T cell line Molt-4 and Jurkat served as controls. RESULTS: Only 38.78%?16 26% of 24 V? subfamily T cell were selectively expressed in cord blood, predominantly in V? 3, 5, 8, 9 and 13, whereas all 24 V? subfamilies could be detected in T cells from peripheral blood of normal individuals. Genescan analysis showed that all PCR products of TCR V? subfamilies from cord blood or normal individual peripheral blood displayed multi-peaks. CONCLUSION: Some TCR V? subfamily T cells were absent in cord blood. All TCR V? subfamily T cells in cord blood displayed polyclonality.
