Effects of sevoflurane and anoxia induced preconditioning on survival and apoptosis of neonatal rat orprdiac myocytes
- VernacularTitle:七氟醚和缺氧预适应对乳鼠心肌细胞生存和凋亡的影响
- Author:
Ying TANG
;
Quanyun WANG
;
Yingqiu MAO
- Publication Type:Journal Article
- Keywords:
Ischemia preconditioning;
Myocardium;
Cytology;
Cell hypoxia;
Cell survival;
Apoptosis;
Sevoflurane
- From:
Chinese Journal of Anesthesiology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the difference between sevoflurane- and anoxic preconditioning in protecting newborn rat heart muscle cells from anoxia-reoxygenation injury. Methods The second generation of primary cultured cardiac myocytes from 2-3d newborn SD rats were randomly divided into 4 groups: control group (C), anoxia/reoxygenation group (A/R) in which cultured cardiac myocytes were exposed to 2h anoxia followed by 48h reoxygenation; anoxic preconditioning group (IP) in which before A/ R the cardiac myocytes were pretreated with 20 min anoxia; sevoflurane preconditioning group (S) in which cardiac myocytes were pretreated with 20 min 2.5% sevoflurane (1.5 MAC) before A/R. Ultrastructure of heart muscles cells was observed 1 h after reoxygenation, Cell survival was determined by MTT rapid colorimetric assay and apoptosis was measured by flow cytometry at 0, 1, 12, 24, 36 and 48 h after reoxygenation. Results (1) In S and IP group there was no significant change in ultrastructure and no apoptosis cell was found, whereas in A/R group the change in ultrastructure was significant , and apoptosis cells were found. (2) The cell survival in group S and group IP was significantly lower than that in group C but significantly higher than that in group A/R. (P 0.05) . The survival of cardiac myocytes increased with prolongation of reoxygenation time in group S and group IP. (3) The apoptosis percentage of cells in group S and group IP was significantly higher than that in group C and lower than that in group A/R(F
