Determination of cocaine in rat's serum by high performance liquid chromatography
- VernacularTitle:高效液相色谱法测定小鼠血清中可卡因的含量
- Author:
Lin SUN
;
Jun SONG
;
Wei HE
- Publication Type:Journal Article
- Keywords:
Toxicological analysis;
Cocaine;
Serum;
HPLC;
UV detection.
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an assay method for quantitative analysis of cocaine in rat's serum by high-performance liquid chromatography (HPLC). Method Serum samples (30?l) were extracted with 2% isoamyl alcohol and hexane. The organic phase was extracted with 0.1 mol/L HC1. The quantitative analysis was achieved by using a 5?m reversed-phase Kromasil CIS column with mobile phase of 0.04mol/ L phosphate buffer solution (containing 0.26 mmol/L tetrabutylammonium chloride , pH3.2) - acetonitrile (83:17). The wave length of the ultraviolet detection was performed at 235nm. Results The lowest limit of the detection limit was 25ng/ml. The recovery of the cocaine was 71 %. Conclusion The method is sensitive, simple and easy to perform.
