Determination of Resibufogenin and Cinobufagin in Meihuadianshe Pills by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的含量
- Author:
Yafang LI
- Publication Type:Journal Article
- Keywords:
Meihuadianshe pill;
resibufogenin;
cinobufagin;
RP-HPLC;
determination of content
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for determination of the contents of resibufogenin and cinobufagin in Meihuadianshe pills METHODS:The condition of HPLC was Kromasil C18 column,acetonitrile-0 5%KH2PO4(50∶50) as a mobile phase and detecting wavelength 296nm RESULTS:This method showed good linearity The average recoveries of resibufogenin and cinobufagin were 97 40% with a RSD of 2 2% and 97 78% with a RSD of 2 6% respectively CONCLUSION:The method is simple,reliable and satisfactory in resolution It can be used in quality control of Meihuadianshe pills