The preliminary study on commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats
- VernacularTitle:SD大鼠视网膜神经细胞培养上清液对胚胎干细胞体外分化的诱导作用
- Author:
Zhikuan YANG
;
Jian GE
;
Yan GUO
- Publication Type:Journal Article
- Keywords:
Embryonic induction;
Cell differentiation;
Neuron;
Stem cells;
Animal testing alternatives
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats. Methods The medium from cultured retinal neurons of SD rats were collected, sterilized and mixed with DMEM medium according to 2∶3 proportion, ES cells were cultured with these mixed medium and were observed under the phase contrast microscope daily, the induced cells were identified by NF immunohistochemistry methods. Results The ES cells cultured with these mixed medium can differentiate into neuron like structure, and the induced cells were positive in NF immunofluorescence staining. Conclusion The medium from cultured retinal neurons of SD rats can induce ES cells commitment differentiation into neuron like structure.
