Effect of lipopolysaccharide on cytosolic Ca~(2+) and Gq protein in pulmonary microvascular endothelial cells of rats and the interferring action of anisodamine
- VernacularTitle:脂多糖对大鼠肺微血管内皮细胞[Ca~(2+)]_i和Gq蛋白的影响及山莨菪碱的干预
- Author:
Chuanbiao FANG
;
Gengyun SUN
;
Li BAI
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Calcium;
Gq protein;
Endothelium;
Cells;
Lung;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of lipopolysaccharide(LPS) on cytosolic Ca 2+ and Gq protein in pulmonary microvascular endothelial cells of rats (RPMVECS) and the interferring action of anisodamine.METHODS: RPMVECS of Wistar rats were isolated, cultured and identified. The concentration of cytosolic Ca 2+ and Gq protein were measured by Fura-2/AM and flow cytometry, respectively.RESULTS: (1) Compared with the control group, the level of cytosolic Ca 2+ increased and the level of Gq protein decreased significantly at 30 min and 90 min after stimulation of LPS;(2) Increase in cytosolic Ca 2+ and decrease in Gq protein in RPMVECS were inhibited by anisodamine. CONCLUSION: LPS induced the increase in cytosolic Ca 2+ and the decrease in Gq protein in RPMVEC, which was inhibited by anisodamine.
