Signal transduction and mechanisms of integrin CD18 expression regulated by PMA
- VernacularTitle:PMA对U937细胞CD18 mRNA表达的诱导作用及其机制
- Author:
Xiaowen HE
;
Yujian LIU
;
Zhen PAN
;
Jigen ZHONG
;
Jia LU
- Publication Type:Journal Article
- Keywords:
Phorbol esters;
Integrins;
NF-?B;
RNA,messenger
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of PMA(phorbol 12-myristate 13-acetate) on the expression of CD18 and the mechanism. METHODS: The technique of quantitative RT-PCR analysis was used to measure the expression of CD18 mRNA in U937 cells treated by PMA. RESULTS: PMA could significantly induce CD18 mRNA expression in a dose and time-dependent manner. The induction effects of PMA on CD18 mRNA could be inhibited obviously by Myr (2 ?mol/L), a specific inhibitor of PKC, and APDC, an inhibitor of NF-?B, but not be inhibited by curcumin, a inhibitor of transcriptional factor AP-1. CONCLUSION: PMA enhanced the expression of CD18 via the pathway of PKC. Transcriptional factor NF-?B, but not AP-1, was essential for the gene transcription of CD18 in U937 cells treated by PMA.
