Effects of insulin on cardiac fibroblast proliferation and cardiac myocyte hypertrophy
- VernacularTitle:胰岛素促进心肌成纤维细胞增殖和心肌细胞肥大的作用
- Author:
Huashan HONG
;
Lan LIN
;
Yibo WANG
;
Qiong JIANG
;
Jianhua CHEN
- Publication Type:Journal Article
- Keywords:
Insulin;
Myocardium;
Fibroblasts;
Hypertrophy
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of insulin on proliferation and hypertrophy of cardiac myocytes and its role in the induction of cardiac hypertrophy. METHODS: 1. The neonatal rat cardiac myocytes and cardiac fibroblasts were cultured respectively and identified with light microscopy, electron microscopy and immunocytochemistry. 2. Cell proliferation was measured with cell number, metabolic activity and DNA synthesis (with WST-1, BrdU enzyme-linked immunosorbent assay ) and the percentage of S+G 2+M in cell cycle (by flow cytometry ). 3.Cell hypertrophy was evaluated by cell protein content (Coomassie Briliant Blue's method). RESULTS: 1. The cultured cells showed the characteristic of cardiac myocytes and cardiac fibroblasts, respectively. 2. After being treated with insulin, the cell number, absorbance of BrdU incorporation and WST-1 cleavage products and the percentage of S+G 2+M of cardiac fibroblasts increased significantly ( P 0.05). 3. Protein content of cardiac myocytes increased significantly in a dose-dependent manner ( P
