Effect of IL-12 on the expression of Fas/FasL and TNF?
- VernacularTitle:IL-12诱导T细胞凋亡对Fas/FasL和TNFR/TNF?表达的影响
- Author:
Yuansheng LIU
;
Hongtao FAN
;
Qiuye GUO
;
Xiuzhi GUO
;
Guangxiao TAN
;
Peng CHEN
;
Tao ZHOU
;
Xiaoyi CHEN
- Publication Type:Journal Article
- Keywords:
Interleukin-12;
Leukemia, T-cell;
Apoptosis;
Annexin V;
RNA, messenger
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of IL-12 on T lymphocytes apoptosis, the expression of Fas/FasL and TNFR/TNF?. METHODS: Terminal dUTP nick end labeling(TUNEL) and Annexin V assay were used. Anti-TNFR were labeled with FITC, anti-CD95 was labeled with PE and Anti-FasL with biotin. Three kinds of T cells (HTB176,TIB152 and human normal T cells) were analysed through flow cytometry. RESULTS: At 1st hour after being treated with IL-12, the expression of FasL protein and FasL mRNA in HTB176 and TIB152 began to increase and reached peak value in 24 hours. In the normal T cells, FasL just began to increase in 1 hour and maintained stability in 6, 12 and 24 hours through the later experiment period. All three kinds of T cells displayed no change in the expression of CD95 and TNFR/TNF? under the stimulation of IL-12. CONCLUSION: Expression of such apoptosis regulating factors were different in the apoptosis of T cells induced by IL-12.
