Influences of protein kinase C inhibitors on the expression of IL-2 and IFN-?by human T-lymphocytes
- VernacularTitle:蛋白激酶C抑制剂对T细胞表达IL-2及IFN-?的影响
- Author:
Xianhui HE
;
Yaoying ZENG
;
Lihui XU
;
Hong SUN
;
Zhen LI
;
Jingfang DI
- Publication Type:Journal Article
- Keywords:
Gossypol;
Protein kinase C;
Interleukin-2;
Interferons
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influences of protein kinase C(PKC) inhibitors on the expression of interleukin-2(IL-2) and interferon-?(IFN-?) by in vitro activated T-lymphocytes. METHODS: Double fluorescent staining together with flow cytometry was adopted to detect intracellular cytokines and to analyze the effects of H7 and gossypol on IL-2 and IFN-? expression levels of T-lymphocytes stimulated with phorbol ester (PDB)+ionomycin(I) in the presence of monensin.RESULTS: The expression rates of IL-2 and IFN-? of CD3 + T cells stimulated with PDB+I for 4 h were 16.64?2.04 and 25.81?3.53( ?s ), respectively, which were significantly higher than that of control (1.06?0.22 and 3.12?0.77)( P
