The effects of hypertonic-hyperoncotic solution on the lipopolysaccharide-stimulated ICAM-1 mRNA expression in human vascular endothelial cell
- VernacularTitle:高晶体-高胶体渗透压混合液对脂多糖刺激的人血管内皮细胞ICAM-1mRNA表达的影响
- Author:
Shiying YUAN
;
Xueping ZHANG
;
Bangxiong ZENG
- Publication Type:Journal Article
- Keywords:
Hypertonic solutions;
Osmotic pressure;
Endothelium, vascular;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Anesthesiology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alteration in intercellular adhesion molecule (ICAM)-l mRNA expression on the surface of human vascular endothelial cells (HUVECs) induced by LPS stimulation and the effects of hypertonic-hyperoncotic solution (HHS) . Methods HUVECs were enzymatically isolated and cultured in RPMI 1640 culture medium at 37℃ for about 7 days when a monolayer of endothelial cells has grown. The primarily cultured HUVECs were mixed with LPS 100ng/ml and incubated at 37℃ for 30min, 1,4,8, 12and 24 h. 10%NaCl and 10% hydroxyethyl starch (Fresenius) were added to RPMI 1640culture medium. The HHS concentrations were 0.25% and 0.5% respectively. HUVECs were first incubated with either HHS (0.25% , 0.5% ) or isotonic solution for 10 min, 1h and 4h and then stimulated by LPS for 4h. The ICAM-1 mRNA was measured by reverse transcriptase-PCR. Results There was slight expression of ICAM-1 mRNA on the surface of HUVECs in normal condition. ICAM-1 mRNA expression began to increase at 1h exposure to LPS and reached peak value at 4h. Both 0.25% and 0.5% HHS could prevent the LPS-induced ICAM-1 mRNA up-regulation (P
