Expression and mutation of ING1 in colorectal carcinoma and their clinical significance
- VernacularTitle:抑癌基因ING1 mRNA在大肠癌中的表达、突变分析及其意义
- Author:
Ziqiang WANG
;
Zhimin CAI
;
Peiwu YU
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Tumor suppressor genes;
ING1;
Mutation
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the expression of ING1 mRNA and its mutation status in human colorectal cancer. MethodsSamples of normal mucosa and cancer tissues from 52 patients with colorectal cancer were detected for their expression of ING1mRNA with RT PCR, and screened for its mutation with PCR SSCP. The expression of p53 protein was detected with immunohistochemical staining.ResultsThe impaired expression of ING1mRNA in colorectal cancer was associated with an advanced Dukes′ stage and metastasis of lymph nodes ( P ≤ 0 01). The expression of ING1mRNA positively correlated to that of p53 protein ( P ≤0 01). And a infrequent mutation of ING1 was also observed. Conclusion Our results suggest that ING1 contributes to the tumorigenesis and progression of colorectal cancer through down regulating its expression rather than through mutation.
