Effects of heparin on staurosporine-mediated cell proliferation and apoptosis in human nasopharyngeal carcinoma CNE2 cells
- VernacularTitle:肝素对叉孢素介导的人鼻咽癌CNE2细胞的增殖与凋亡的影响(英文)
- Author:
Hongliang LI
- Publication Type:Journal Article
- Keywords:
Heparin;
Apoptosis;
Staurosporine;
Protein kinase C
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine whether heparin combined with staurosporine was more effective than either agent alone on anti-proliferation and apoptosis in CNE2 cells, to improve the understanding of the mechanisms by which staurosporine and heparin influence cell proliferation and apoptosis, and to further examine the role of different apoptosis-related oncogenes in regulation of staurosporine and/or heparin -mediated cell proliferation and apoptosis in CNE2 cells. METHODS: Cell viability was determinted by methl-terazolium (MTT). Apoptosis was evaluated by Hoechst 33258 staining, TUNEL, and agarose gel electrophoresis as well as flow cytometry. The expression of bcl-2, bax, c-myc was examined by RT-PCR and Western blot. RESULTS: Staurosporine elicited typical apoptotic morphologic changes. Compared with staurosporine alone, staurosporine plus heparin obviously enhanced the number of TUNEL-positive cells,and the percentage of apoptosis was 2 6%?1 2%, 14 3%?1 5%, 33 2%?0 2%, 57 9%?0 3%, respectively. SubG 1 peaks were detected on flow cytometry, the apoptotic index was enhanced from 3 8% to 56 8%. The DNA laddering was more clearly observed by the treatment with staurosporine plus heparin. Similarly, the expression of bax protein and mRNA, and the level of c-myc protein were overexpressed by the treatment with staurosporine plus heparin, while no significant changes were found in the expression of bcl-2 protein and mRNA following drug exposure. CONCLUSION: The present data suggested that heparin and staurosporine appears to interact in a synergistic manner, which may be regulated by the overexpression of c-myc, bax.
