Effect of OX-LDL and simvastatin on PKC activity and cytosolic free Ca~(2+) in cultured rat aortic smooth muscle cells
- VernacularTitle:OX-LDL及辛伐他汀对平滑肌细胞PKC活性和胞内Ca~(2+)的影响
- Author:
Jinchuan YAN
;
Zonggui WU
;
Lingzhen ZHANG
;
Li LI
;
Jie FAN
;
Ling LING
;
Wenyu HAN
;
Suolon ZHANG
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Simvastatin;
Muscle, smooth;
Cells;
Protein kinase C;
Calcium
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AMI: To clarify whether OX-LDL and simvastatin can induce the changes of PKC activity and cytosolic free Ca 2+ in rat aortic smooth muscle cells (ASMC). METHODS: PKC activity and cytosolic free Ca 2+ were measured by its ability to transfer phosphate from ATP to lysine-rich histone and flow cytometric analysis after loading with the Ca 2+ dye fluo 3/Am, respectively. RESULTS: OX-LDL increased PKC total activity in a dose-dependent manner and induced translocation of PKC from the cytosolic to membrane, while OX-LDL induced biphasic [Ca 2+ ]i responses including the rapid initial transient phase and the sustained phase. When simvastatin was added, the translocation of PKC was markedly decreased and simvastatin did not impair the initial peak response to OX-LDL but significantly reduced the subsequent plateau phase. CONCLUSSION: OX-LDL can induce dynamic changes of signal transduction of PKC and cytosolic free Ca 2+ in ASMC and these two events are closely linked.
