Expression of the Fas antigen and induction of apoptosis in human eosinophils in vitro
- VernacularTitle:嗜酸细胞Fas抗原表达及其在凋亡诱导中的作用
- Author:
Kefang LAI
;
Xiaoming GUO
;
Changzheng WANG
;
Xianjian GUO
;
Guishen QIAN
- Publication Type:Journal Article
- Keywords:
Eosinophils;
Apoptosis;
Antigens;
Interlukin-5
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The expression of the Fas Antigen and induction of apoptosis by anti-Fas antibody in esoinophils in vitro were investigated. METHODS: Purified eosinophils from health donors were cultured for 72 h in the presence of human IL-5 and with or without anti-Fas monclonal antibody (MoAb) at various concentrations (1-1000 ?g/L). The expression of the Fas antigen on eosinophils was determined by immunocytochemistry. The changes of eosinophils viability and apoptosis were also studied. RESULTS: The Fas antigen was expressed on freshly isolated eosinophils, which had no significant changes after culture in the presence or absence of IL-5. The anti-Fas MoAb at different concentration suppressed significantly the IL-5-mediated eosinophils survival (78%?9%). When eosinophils were cultured in the presence of IL-5 (1?10 4 U/L) with anti-Fas MoAb (1 000 ?g/L), the percentage of alive cell decreased to 30%?12%( P
