Effect of cold acclimation on GTP binding to uncoupling protein in mitochondria inner membrane of rat brown fat
- VernacularTitle:冷适应对大鼠棕色脂肪组织线粒体内膜解偶联蛋白与嘌呤核苷三磷酸结合的影响
- Author:
Xiping TUO
;
Zhenchun MIAO
;
Dajin ZOU
;
Xuemi WANG
- Publication Type:Journal Article
- Keywords:
Cold;
Brown fat;
Uncoupling protein;
Guanosine triphosphate
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the effect of cold acclimation on activity and concentration of UCP in mitochondria inner membrane of rat brown fat. METHODS: Using -labeled GTP binded UCP in mitochondria, radioactivity was measured by liquid aglimmer instrument, the dissociation constand(Kd) and maximal binding(Bmax) were determined by Scatchard plot. RESULTS: In cold acclimation pretreated rats, brown fat weight increased, Bmax increased 1.9-fold,Kd decreased 1.4-fold compared with control. CONCLUSION: Cold acclimation not only increase concentration of UCP in mitochondria inner membrane of rat brown fat,but also increase its activity.
