Effects of lovastatin on ras expression and p21~(Ras) membrane localization in human promyelocytic leukemia NB4 cells in vitro
- VernacularTitle:洛伐他汀对NB4细胞ras基因p21~(Ras)膜结合作用影响的体外试验
- Author:
Feng GUO
;
Jiannong CEN
;
Zixing CHEN
;
Wei WANG
;
Jianxin FU
;
Xiaowei YANG
- Publication Type:Journal Article
- Keywords:
Genes, RAS;
Lovastatin;
Proto-oncogene protein P 21 (RAS)
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of cholestoral and mevalonate synthesis inhibitor lovastatin (LOV) on the proliferation of NB4 cells and elucidate the mechanisms. METHODS: Cell proliferation was analyzed by MTT assay;the expression of H, K, N- ras oncogenes in NB4 cells at different time point after LOV treatment were determined by semi-quantitative RT-PCR. Both total p21 Ras protein and p21 Ras protein on the cellular membrane were examined by flow cytometry. RESULTS:①LOV inhibited the proliferation of NB4 cells. ②All three kinds of ras were expressed in NB4 cells. ③LOV caused no increase in H, K, N- ras mRNA level. Amount of total p21 Ras protein did not change as the time varied. Concomitantly,p21 Ras protein localized on the cellular membrane decreased. CONCLUSION:LOV inhibits the proliferation of NB4 cells. Targeting HMG-CoA reductase, LOV blocks the isoprenylation of p21 Ras protein which affects its anchorage on the cellular membrane. No change in the H, K, N- ras mRNA and total p21 Ras protein expression is detected.
