Establishment of human herpesvirus 6B U83 transgenic mouse lines
- VernacularTitle:人类疱疹病毒6B U83转基因小鼠系的建立
- Author:
Lifa ZHANG
;
Yonghuang TANG
;
Pin ZOU
- Publication Type:Journal Article
- Keywords:
Herpesvirus-6, human;
Mice, transgenic;
U83 fragment
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Human herpesvirus 6B(HHV-6B) is the etiologic agent of exanthem subitum and is associated with febrile illnesses in children, neurological manifestations during primary infection, and clinical complications in organ transplant patients. The ORF U83 of HHV-6B has been identidied encoding a functional chemokine and U83 protein was detected out in brain tissues of some patients with multiple cirrhosis. The aim of this study was to establish HHV-6B U83 transgenic mouse lines, which would provide a pathological animal model for the research of the relationship between HHV-6B U83 gene and multiple cirrhosis. METHODS: Transgenic animal technique was applied in the present study. RESULTS: We successfully established 3 lines of transgenic mice with HHV-6B U83 gene integrated to their chromosomes, through constructing the DNA fragment which consisted of GFAP promotor, HHV-6B U83 and mouse protamine 1 sequence and injecting to the mouse eggs. CONCLUSION: This establishment of HHV-6B U83 transgenic mouse lines would benefit the observation and analysis of association of HHV-6B infection with neurological system diseases.
