Effect of iNOS on renal injury in dietary hypercholesterolemia rats
- VernacularTitle:诱生型一氧化氮合酶的变化在高胆固醇血症大鼠肾损害中的作用
- Author:
Hongyan LIU
;
Ruhan JIA
;
Guohua DING
;
Congxin HUANG
- Publication Type:Journal Article
- Keywords:
Hypercholesterolemia;
Nitric oxide;
Nitric oxide synthase;
Apoptosis;
Proteinuria
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between change of inducible nitric oxide synthase (iNOS) and rat renal damage in dietary hypercholesterolemia. METHODS: High cholesterol (HC) group was fed with diet supplemented with 4% cholesterol and 1% cholic acid for 8 weeks. Another group was fed with normal diet. Expression of iNOS at protein level was evaluated by immunohistochemical staining and iNOS mRNA levels in renal cortex by reverse transcription-polymerase chain reaction (RT-PCR). The apoptotic cells in renal tissue at 8th week were evaluated by TUNEL method. RESULTS: Total serum cholesterol and LDL-cholesterol levels in HC group were higher than that in control group ( P
