Expression and localization of midkine in hepatocellular carcinoma
- VernacularTitle:中期因子转录物及其蛋白在肝细胞癌中的定位与表达研究
- Author:
Xiangji LUO
;
Zhengfeng YIN
;
Xiaoyan KANG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Biological factors;
In situ hybridization;
Immunohistochemistry
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression and localization of midkine (MK) in human hepatocellular carcinoma. MethodsIn situ hybridization and immunohistochemical analysis for MK were performed on samples of both tumor tissues and paratumor tissues from HCC and benign liver tumors. ResultsThe distribution and localization of the MK transcripts′ signals determined by in situ hybridization were similar to those obtained by immunohistochemical analysis. Most HCC tissues showed enhanced positive reaction within cytoplasm to both MK probe and MK immunostaining. There was no significant difference in clinicopathological parameters between MK negative and positive cases of HCC. ConclusionsHuman hepatocellular carcinoma overexpresses MK at the mRNA and protein level.
