Inhibition of telomerase activity of colorectal cancer cells by chemotherapeutic drugs
- VernacularTitle:化学治疗药物对大肠癌细胞端粒酶活性的影响
- Author:
Xiaoming JU
;
Wenhuai XU
;
Yuanlian WAN
- Publication Type:Journal Article
- Keywords:
Chemotherapy, adjuvant;
Telomerase;
HT-29 cells;
Cisplatin
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the inhibition of telomerase activity of colorectal cancer by chemotherapeutic drugs.MethodsBy using telomerase repeat amplification protocol (TRAP) combined with PAGE silver staining, we detected the telomerase activity of human colorectal cancer cell line HT-29 under the effect of cisplatin, doxorubicin, pirarubicin, mitomycin C, and 5-FU.ResultsIn high doses,no inhibition of tolemerase activity was found when cells were collected after only 4 hours of drug treatment, but the telomerase activity was completely inhibited by cisplatin when the drug was removed and cells were reculured for 20 hours. However, doxorubicin, pirarubicin, mitomycin C, 5-FU had no such effect. ConclusionCisplatin inhibits telomerase activity of human colon cancer cell HT-29, while other drugs had no such effect.
