The influence on the quantity of the template DNA on the results of the automatic fluerescent detection of STR multiplex amplification
- VernacularTitle:模板DNA用量对荧光STR复合扩增检测的影响
- Author:
Lihua GU
;
Yuan PING
;
Li CHENG
- Publication Type:Journal Article
- Keywords:
STR;
Optimal amount of template DNA;
Profiler Plus Kit;
3100 Genetic Analyzer
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective For the purpose of exploring the influence exerued by the amount of template DNA on the results of automatic fluerescent detection. Method For the purpose of buiding the optimal amount of template DNA amplified, we have analyzed different amount of template DNA (9947A) by 3100 Genetic Analyzer with the multiplexing Profiler Plus Kit. Results The optimal amounts of template DNA amplified were between 0.31ng and 2. 5ng by using 3100 Genetic Analyzer. Conclusion It will produce the incorrect analytic results if the amounts of template DNA were too high or too low.
