Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells
- VernacularTitle:金属硫蛋白抑制同型半胱氨酸诱导的大鼠血管平滑肌细胞增殖
- Author:
Jun CAO
;
Zhisheng JIANG
;
Yongfen QI
;
Lin GAO
;
Chaoshu TANG
;
Junba DU
- Publication Type:Journal Article
- Keywords:
Metallothionein;
Homocysteine;
Muscle, smooth, vascular;
Cells;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS: VSMCs proliferation was measured by -TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by [ 109 Cd]-hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS: Hcy(10 -6 -10 -4 mmol/L) stimulated -TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, -TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold ( P
