Anti-proliferative effect of arotinoid Ro40-8757 on human pancreatic carcinoma cell line JF-305
- VernacularTitle:维甲类化合物Ro40-8757对人胰腺癌JF-305细胞株的抗增殖作用及其机制的研究
- Author:
Yang SU
;
Kejian GUO
;
Yulin TIAN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Cell cycle;
Cell cycle proteins;
Arotinoids
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the anti-proliferative effect and the mechanism of arotinoid Ro40-8757 on human pancreatic carcinoma cell line JF-305.MethodsMTT assay was used to evaluate the growth inhibition effect of Ro40-8757 on human pancreatic carcinoma cell line JF-305. The expression of p27 protein was measured by Western blotting, and flow cytometry for cell cycle analysis.ResultsRo40-8757 significantly inhibited the growth of JF-305 cells with a dose-, time-dependent manner. After 72 hours treatment with 10 -5mol/L Ro40-8757, the proportion of cells in G 1 phase increased from 56% to 76% while the cells in S phase decreased from 25% to 7%. Western blot analysis showed that the expression of p27 protein in cells treated with Ro40-8757 increased markedly.Conclusion Ro40-8757 inhibits the growth of pancreatic carcinoma cell line JF-305 possibly by up-regulating p27 protein expression and arresting cell cycle.
