Determination of the Concentration of Rhein in Plasma by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定大鼠血浆中大黄酸的含量
- Author:
Qizhi LU
- Publication Type:Journal Article
- Keywords:
rhein;
plasma concentration;
RP-HPLC
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for determining rhein in plasma.METHODS:Column:Spherisorb C18(4.0mm?250mm,5?m),Mobile phase:0.1% of phosphoric acid(pH 3.0)-methanol(30∶70,V/V),Flow rate:1.0ml/min;Detection wavelength:435nm.RESULTS:Rhein was completely separated and no interference was found.The peak areas of rhein within the range between 0.032～4.8?g/ml showed good linearity of the concentrations,r=0.9 990.The detection limit of rhein in plasma was 16ng/ml.The recovery rate was 92.09%～98.53%.The within-day RSD was 1.65%～6.68%,and between-day RSD was 4.93%～10.01%.CONCLUSION:This method is simple and sensitive,and can be used for analysing rhein-containing biological samples.
