Assay of Concentration of Isoniazid in Plasma With HPLC
- VernacularTitle:高效液相色谱法测定异烟肼的血药浓度
- Author:
Feng QIU
;
Limei ZHAO
;
Guifeng ZHANG
;
Shanbin GUO
- Publication Type:Journal Article
- Keywords:
isoniazid;
HPLC;
plasma
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:A HPLC method has been developed to determine the concentration of isoniazid in plasma.METH_ODS:The Eclipse XDB-C18 column was used as fix phase and acetonitrile-0.05mol/L ammonium dihydrogen phosphate as mo_bile phase,detection wavelength was 280nm.The plasma sample was injected directly for determination after being deproteinized with 10% trichloroacetic acid and reacted with cinnamaldehyde and abstracted with ether.RESULTS:Good linear relationship was shown from 0.10 to 12.0?g/ml and the averge recovery of isoniazid was 95%～105%.CONCLUSION:The method is rapid,sensitive and is rarely interfered so it can be used in study of pharmacokinetics of isoniazid.
