Determination of the Concentration of Midazolam in Plasma by RP-HPLC-UV Detection
- VernacularTitle:反相高效液相色谱-紫外法测定血浆中咪达唑仑浓度
- Author:
Meihua CAI
;
Shanjuan WANG
;
Yannan HANG
- Publication Type:Journal Article
- Keywords:
midazolam;
HPLC;
ultraviolet detection
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To build up a method of determining the concentration of midazolam in plasma by RPHPLC-UV detection.METHODS:The separation was carried out by a reversed-phase Hypersil ODS column(250mm?4.0mm,5?m) with a mobile phase consisting of methanol-acetonitrile-0.02mol/L potassium phosphate buffer(pH 7.4) (65∶25∶10,V/V).Mida_zolam was extracted from alkalinizing plasma and soluted in the mobile phase then detected at 221nm.RESULTS:The calibration curve had the fine linearity in the concentration range 50～1 600ng/ml(r=0.9 999).The detection limit was 2ng/ml.The absolute recovery was 90.8%～95.4%,the relative recovery was 99.3%～101.3%.The within-day and between-day precision(CV%) was 1.94%～5.16%,3.00%～6.39% respectively.CONCLUSION:The method is simple,stable and highly sensitive and could meet with the research of clinical pharmacokinetics.
