To induce uveal melanoma cells apoptosis by mixed structure bcl-2 antisense oligodeoxynucleotides multidrug resistance
- VernacularTitle:嵌合型bcl-2反义寡核苷酸对体外培养葡萄膜黑色素瘤细胞存活的影响
- Author:
Linjie GUO
;
Zhongyao WU
;
Sheng ZHANG
- Publication Type:Journal Article
- Keywords:
Uveal neoplasms;
Melanoma;
Oligonucleotides;
In vitro;
Cell death
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of anti apoptosis gene bcl 2 in the survival of cultured uveal melanoma UM cells. Methods Antisense oligodeoxynucleotides (AS ODN) bcl 2 were delivered with cationic lipid to primary cultured UM cells. The inhibitory effect of AS ODN bcl 2 on proliferation of UM cells was examined by 3, 4,5 Dimethyliazol 2,5 diphenyl tetrazolium bromide (MTT) method. Using DNA ladder to determine if the UM cells had been apoptotic. Bcl 2 expression was detected by RT PCR and Westernblot technics. Results The effect of AS ODN bcl 2 in inhibiting the proliferation of cultured UM cells had opposite relation to dosage. It down regulated the mRNA and protein level of bcl 2 gene, and the sense ODN didn′t have this effect. Conclusion AS ODN bcl 2 can down regulate bcl 2 expression, inhibits UM cells proliferation and induces apoptosis.
