Distribution of free Ca~(2+) and characterization of Ca~(2+) channel in the cultured early-stage human embryonic retina
- VernacularTitle:培养的胚胎视网膜神经细胞Ca~(2+)分布与Ca~(2+)通道特征
- Author:
Wenwen LIU
;
Ping XU
;
Qiangsu GUO
- Publication Type:Journal Article
- Keywords:
Neurons/physiology;
Retina/metabolism;
Embryo;
Cell cullture
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of free Ca 2+ and characterization of Ca 2+ channel in the cultured 11～15 week human embryonic retina. Methods The cells from 11～15 week embryonic retina were dissociated enzymatically and cultured on coated cover slides. Three weeks later, Fluo3, a Ca 2+ indicator, was added in Ca 2+ containing or no Ca 2+ Hepes buffer and incubated with retinal cells for 30 min, meanwhile with or without verapamil, perdipine, or dexamethasone adding. Ca 2+ staining and Ca 2+ transit before and after 10 ?mol/L or 50 ?mol/L KCl stimulation was observed and recorded using Zeiss LM510 confocal laser scanning microscopy. Results In response to K + exposure, a rapid rise of free Ca 2+ in cytoplasma and nuclei of neuron and glial cell was observed, but was repressed with the treatment of verapamil, perdipine, or dexamethasone. The obvious Ca 2+ influx from cytoplasma into nuclei was observed even in the absence of external Ca 2+ . Conclusion L type Ca 2+ channel was expressed and functionally matured in the cultured early stage human embryonic retina.
