Interleukine 1 receptor antigonist inhibits proinflammatory factor production by human retinal pigment epithelial cells in vitro
- VernacularTitle:白介素1受体拮抗剂抑制视网膜色素上皮细胞炎前因子的表达
- Author:
Zongming SONG
;
Yannian HUI
;
Jia QU
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye/immunology;
Interleukines/diagnostic use;
Retina
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate production of interleukine 6 (IL 6) and interleukine 8 (IL 8) by retinal pigment epithelial (RPE) cells and its inhibition by interleukine 1 receptor antigonist(IL 1ra). Methods Cultured human RPE cells was treated with interleukine 1 ? (IL 1?, 10 ng/ml) and/or IL 1ra ( IL 1ra, 1、10、100 ng/ml). IL 6 and IL 8 mRNA and protein expression were detected by ELISA, immunohistochemistry and in situ hybridization (ISH) assay. Results IL 6 and IL 8 in conditioned media of RPE cells in controls was 2 000 pg/ml and 5 000 pg/ml respectively after stimulation of IL 1? for 8 h. IL 1ra (100 ng/ml) significantly inhibited IL 6 (300 pg/ml, t=8.011, P
