Cultivation of rabbit iris pigment epithelial cells and observation of its ultrastructure
- VernacularTitle:兔眼虹膜色素上皮细胞的组织培养与超微结构观察
- Author:
Zhaoyan WANG
;
Yannian HUI
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye/ultrastructure;
Iris;
Tissue culture
- From:
Chinese Journal of Ocular Fundus Diseases
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for primary culture of iris pigment epithelial cells (IPE). Methods Enzyme Assisted microdissection was used to isolate and cultivate the IPE cells. An identification was made with microscopic and immunohistochemical observations. Results IPE were successfully cultured and showed on differences with RPE in primary culture and subculture. Conclusion Enzyme Assisted microdissection is a reliable and quick method for the isolation of IPE.
