Change of Blood Parathyroid Hormone Level in Hypoparathyroidism Treated by pcDNA3.1?PTH
- VernacularTitle:pcDNA3.1?PTH治疗甲状旁腺功能低下症血液甲状旁腺激素变化
- Author:
Jimin WEN
;
Weihui ZHANG
;
Chun SONG
- Publication Type:Journal Article
- Keywords:
Parathyroid hormone;
Gene therapy;
Hypoparathyroidism
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Measuring the concentration of PTH when pcDNA3.1?PTH was injected into the muscle of rabbits with hypoparathyroidism (HPT),studying the relationship between change of blood PTH and pcDNA3.1?PTH dosage.Methods Different dosages of pcDNA3.1?PTH(100?g/ml, 300?g/ml and 500?g/ml)containing human PTH gene were injected respectively into muscle of the experimental rabbits after parathyroidectomy in advance.Then the concentration of PTH in blood of rabbits was measured at following different times.Results PTH level began to increase from the first day,which reached in peak concentration at the seventh day after the injection of pcDNA3.1?PTH and persisting two months.Conclusion Human PTH gene was effective in therapying HPT rabbits,their best effective dosages were 300?g/ml and 500?g/ml respectively.
