Study on the potential to differentiate into myocytes of the CD34~+ cells
- VernacularTitle:CD34~+细胞的心肌细胞分化潜能研究
- Author:
Yunxian CHEN
;
Xueyun ZHONG
;
Ruiming OU
;
Huizhen CHEN
;
Weiqiong LUO
;
Liye ZHONG
;
Da XING
;
Zhongcha HAN
- Publication Type:Journal Article
- Keywords:
CD34 + cells;
Myocardial infarction;
Cell differentiation;
Granulocyte colony-stimulating factor;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the potential of differentiatng into myocytes of the granulocyte colony-stimulating factor(G-CSF)-mobilized CD34 + cells. METHODS: Three hours after intraperitoneal injecction of isoprenaline(ISO) to develop acute ischemic model, rats' bone marrow hematopoietic stem cells were mobilized to the site of myocardial infarction by G-CSF. The techniques of immunohistochemisty and HE stain were used to detect the infiltration of CD34 + cells and the regeneration of myocytes in the infarct zones. RESULTS: 24 hours after administration of ISO , a large amount of infiltrative monocytes and regenerative myocytes which were CD34 positive expression could be found in the infarct zones of the G-CSF treatment group, while majority of the infiltrative inflammatory cells in control group were neutrophils and there was no infiltrative cells and myocytes which were CD34 positive expressio, 2 weeks after administration of ISO, there were a plenty of scar in control group, but not in the G-CSF treatment group. CONCLUSION: G-CSF-mobilized CD34 + cells possess the potential to differentiate into myocytes and it may be used in treating acute myocardial infarction.
