Expression of calcium handling protein in diastolic heart failure
- VernacularTitle:舒张性心力衰竭兔Ca~(2+)调控蛋白mRNA和蛋白质的表达
- Author:
Ming ZHONG
;
Yun ZHANG
;
Wei ZHANG
;
Jifeng BIAN
;
Zhao GENG
;
Jin ZHAO
- Publication Type:Journal Article
- Keywords:
Heart failure;
Calmodulin;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To elucidate molecular mechanisms underlying calcium handling protein in diastolic heart failure (DHF) from mRNA level and protein expression, including calcium adenosine triphosphatase (Ca 2+ -ATPase), phospholamban, ryanodine receptor, calsequestrin and L-type calcium channel. METHODS: The mRNA of these calcium handling genes were detected by RT-PCR, and the protein levels were analyzed by Western blot analysis. RESULTS: Compared with sham-operated rabbits, the steady-state levels of mRNA encoding the SR Ca 2+ -ATPase and cardiac L-type calcium channel were decreased significantly in rabbits with DHF, and protein level of SR Ca 2+ - ATPase was greatly reduced, whereas the mRNA and protein levels of other calcium handling protein were unchanged. CONCLUSION: L-type calcium channel and the sarcoplasmic reticular Ca 2+ -ATPase were down regulated in DHF. These changes may be a contributory factor for DHF.
