Three dimensional tissue culture in culture medium with rhbFGF
- VernacularTitle:碱性成纤维细胞生长因子培养条件下的三维组织培养
- Author:
Yifei WANG
;
Yun DAI
;
Jian LIN
;
Zhiying LI
;
Ji LU
;
Meiying ZHANG
;
Jiuxiang LI
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor, basic;
Fibrin;
Adipose tissue;
Macrophages;
Fibroblasts;
Tissue culture
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot or adipose tissue with recombinant basic fibroblast growth factor (rhbFGF). METHODS: MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope were used in the present study. RESULTS: The effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF. CONCLUSION: The blood fibrin clot, with its low immunogenicity, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells.
