Effects of MMP-7asODN on MMP-7 mRNA expression and invasion of KATOⅢ cell
- VernacularTitle:MMP-7反义寡核苷酸对KATOⅢ细胞MMP-7mRNA表达及其侵袭力的影响
- Author:
Yueguang DU
;
Weizhe CHEN
;
Zhi CHEN
- Publication Type:Journal Article
- Keywords:
Matrilysin;
Oligonucleotides, antisense;
Neoplasms;
Cells;
Neoplasm invasiveness
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of MMP-7asODN on MMP-7 expression in human stomach cancer cell line KATOIII and explore the effect of MMP-7 on cancer invasion and metastasis. METHODS: A 15mer PS-asODNs targeted against the MMP-7 mRNA were introduced into KATOIII cells by FuGENE TM 6. The levels of MMP-7 mRNA was observed by reverse transcription polymerase chain reaction (RT-PCR). The ability of the invasion was observed by Boyden Chamber invasion assay. RESULTS: The MMP-7/?-actin ration was 0.31?0.02 for the PS-asODNs treated cells which is significantly lower compared with the control and PS-sODN and PS-mODN( 1.59?0.01, 1.14?0.03,1.51?0.02, respectivly.) P
