Effect of "Bushen Huoxue Xiezhuo" decoction on growth of rat glomerular epithelial cells
- VernacularTitle:补肾活血泄浊汤对体外培养肾小球上皮细胞增殖影响
- Author:
Yue ZHANG
;
Min WEI
;
Fei CHU
;
Qian WANG
;
Jin YAN
- Publication Type:Journal Article
- Keywords:
Epithelium;
Lipopolysaccharides;
Tumor necrosis factor;
Protamines;
Epidermal growth factor-urogastrone;
Drugs, Chinese herbal
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the effect of protamine sulfate, lipopolysaccharide(LPS),tumor necrosis factor(TNF), epidermal growth factor(EGF) and " Bushen Huoxue Xiezhuo" (BHX) decoction on the proliferation of extracorporeal cultured rat glomerular epithelial cells (GEC). METHODS: Their action on the proliferation of rat GEC were investigated using the -TdR incorporation. Meanwhile, the serum of rats treated with BHX decoction was extracted pharmacologically and its effects on the growth of GEC were also studied. RESULTS: LPS, protamine sulfate, TNF-? and EGF could significantly inhibit the -TdR incorporation of GEC in a dose- and time-dependent manner. However, this inhibition could be efficiently reversed by the serum containing BHX decoction. CONCLUSION: GEC is one of the main target cells on which BHX decoction act, and the protection on GEC might be one of the mechanisms underlying the role of BHX decoction in preventing the progression of nephrosis.
