Effects of lipopolysaccharide on hemeoxygenase-1 mRNA and hemeoxygenase-1 protein expression in isolated rat thoracic aorta and pulmonary tissue
- VernacularTitle:脂多糖对离体大鼠胸主动脉和肺组织HO-1mRNA及蛋白表达的影响
- Author:
Yuan LIU
;
Xinmin WU
;
Liping LIU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Aorta, thoracic;
Heme oxygenase(decyclizing);
Gene expression
- From:
Chinese Journal of Anesthesiology
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ve To evaluate the effects of lipopolysaccharide (LPS) on hemeoxygenase-1 (HO-1) mRNA and HO-1 protein expression in isolated rat thoracic aorta (TA) and pulmonary tissue (PT), trying to explain the mechanism of different patho-physiological changes of systemic and pulmonary circulation in septic shock. Methods Twenty-four Wistar rats of either sex weighing 250-400g were sacrificed by cervical spine dislocation. Thoracic aorta and lung tissue were removed under aseptic condition. They were randomly divided into four groups of six animals each: control group (C) and three test groups in which TA and PT were incubated with LPS (1?g?ml-1) for 3h (group LPS3), 8h(group LPS8) and 24h (group LPS24) respectively. HO-1 mRNA expression and HO-1 protein expression in TA and PT were detected by RT-PCR and Western Blot. Results (1) As compared with that in group C, HO-1 protein expression in TA reached peak level in group LPS3, and the peak level was maintained in groups LPS8 and LPS24; while in PT HO-1 protein expression started increasing in group LPS3 and peaked in group LPS8 and tended to decline in group LPS24 but the difference in HO-1 protein expression between group C and LPS 24 was still significant. (2) As compared with that in control group, the HO-1 mRNA expression in TA peaked in LPS3, the peak level was maintained in LPS 8, but it returned to baseline level in LPS 24; while in PT HO-1 mRNA expression started increasing in LPS 3 and peaked in LPS 8 and returned to baseline level in LPS 24. Conclusions LPS can significantly enhance HO-1 mRNA and protein expression in isolated rat thoracic aorta and pulmonary tissue but the time-course is somewhat different between the two tissues. This may explain the different patho-physiologic changes of systemic and pulmonary circulation in septic shock.
