Screening and Cloning the Genes Related to Alopecia Areata by Suppression Subtractive Hybridization
- VernacularTitle:应用抑制性消减杂交技术筛选和克隆斑秃脱发相关基因
- Author:
Zhiqiang SONG
;
Fei HAO
;
Baiyu ZHONG
;
Mingming XIANG
;
Qingchun DIAO
;
Rongqing LIU
- Publication Type:Journal Article
- Keywords:
Alopecia areata;
Papilla, dermal;
Suppression subtractive hybridization
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and clone the genes related to alopecia areata. Methods Dermal papillae of lesional and non lesional follicles were separated from alopecia areata scalp respectively. Suppression subtractive hybridization was used to investigate the difference of expressed genes in dermal papillae of lesional (tester) and non lesional (driver) follicles, and the differentially expressed genes in dermal papillae of lesional follicles were cloned and sequenced. Results A subtractive library of dermal papillae of lesional follicles from alopecia areata was established. A differentially expressed gene in dermal papillae of lesional follicles was successfully cloned and proved to be an autoantigen gene. Conclusions The subtractive library may contain the differentially genes related to alopecia. The autoantigen gene related to alopecia areata need to be further investigated.
