A preliminary study on the quantitative analysis of DNA in the cells of skeletal muscle in rats by image analysis system and the investigation on this method.
- VernacularTitle:大鼠骨骼肌细胞DNA含量变化与死亡时间关系的研究
- Author:
Huijun WANG
;
Qingping ZHENG
;
Guangxun RAO
- Publication Type:Journal Article
- Keywords:
Skeletal muscle, DNA content, PMI,Image analysis
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A quantitative study of the DNA skeletal muscle cell nuclei during 0～96h postmortem was performed.Method By the histochemical and image analysis.Result The color and the areas of the nuclei stained with the Feulgen staining method became fainter and smaller gradually at 12 hpm. There was a linear relationship between the degradation rate of the nuclear DNA and the length of the PMI.Conclusion The quantitative image analysis of DNA has a wide prospect of application, but the method has been studied further in aspects of the precise and standadization of the method as well as the effects exerted on the results from the study materials.
