The analysis of benzodiazepines and its main metabolites by thin-layer chromatography
- VernacularTitle:苯并二氮类药物及其主要代谢物的薄层色谱分析
- Author:
Yuhong WU
;
Jiayi/ TAN
- Publication Type:Journal Article
- Keywords:
benzodiazepines;
thin layer chromatography(TLC)
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A method for the analysis of eleven benzodiazepine in beverages,blood and urine and seven main metabolites of benzodiazepine in urine by solid phase extraction and high performance thin layer chromatography is described in this paper.Method The analytic materials in specimens were extracted by using GDX 101 porous resin as absorbent and diethyl ether as eluant.The extracts were spread out on plates using benzene∶acetone(10∶6) as eluant and were visualized by modified Dragendorffs reagent.Results The absolute sensitivity of the analysis is 0 4 ?g to 0 6 ?g .The limit of detection of benzodiazepines in urine,blood and beverages are 0 4 ?g/ml to 1 0 ?g/ml ,0 6 ?g/ml to 1 0 ?g/ml and 0 4?g/ml to 0 8 ?g/ml respectively.The limits of detection of metabolites of benzodiazepines in urine are 0 4 ?g/ml to 1 0 ?g/ml .Conclusion These results suggested that this method is useful for the detection of benzodiazepines in toxication case.
