Determination of Propofol Concentration in Human Serum With RP-HPLC
- VernacularTitle:反相高效液相色谱-荧光法测定血清中异丙酚浓度
- Author:
Wenli DU
;
Mingxia WANG
;
Yuying XING
- Publication Type:Journal Article
- Keywords:
propofol;
serum concentration of drug;
RP-HPLC
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for determining serum concentration of propofol METHODS:Using ODS C18 column as fixed phase,a mixture of methanol and water(75∶25) as mobile phase,excitation wavelength 270nm,emission wavelength 295nm RESULTS:The linear range was 0 0 375～8 0?g/ml,r=0 9 996 The within-day and between-day RSDs were less than 5%,the average recovery was 83 98% CONCLUSION:This is a good method to monitor propofol serum concentration
