Simultaneous Determination of Estazolam and Diazepam in Serum With HPLC
- VernacularTitle:高效液相色谱法同时测定血清中的舒乐安定和安定
- Author:
Li ZHANG
;
Lin HE
;
Shiming SUN
;
Jing TAN
- Publication Type:Journal Article
- Keywords:
HPLC;
estazolam;
diazepam
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for simultaneous determination of estazolam and diazepam in serum METHODS:The mobile phase consisted of methanol-water-triethylamine-acetic acid(55∶45∶0 5∶0 35)and the ?max was 254nm Phenytoin was adopted as the internal standard The drugs were extracted by diethyl ether under the alkaline condition RESULTS:The calibrating curves of estazolam and diazepam were linear in the ranges of 0 52～38 62?g/ml and 0 50～40 16?g/ml respectively CONCLUSION:The method was appropriate for determination of estazolam and diazepam qualitatively and quantitatively in intoxication accident
