Determination of the Content of Ofloxacin in Compound Ofloxacin Eye Drops by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方氧氟沙星滴眼液中氧氟沙星的含量
- Author:
Shujie JIA
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
ofloxacin;
eye drops;
content determination
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method to determine the content of ofloxacin in compound ofloxacin eye drops METHODS:Using Shim-pack ODS as fixed phase,a mixture to citric acid(0 05mol/L)-acetonitrile-ammonium acetate(0 5mol/L)-0 3% phosphoric acid(78∶22∶1∶3)as mobile phase,and detecting wavelength:293nm,flow rate:1 0ml/min RESULTS:The linear range of ofloxacin was 8 12?g～40 6?g(r=0 9 996,n=5) The average recovery was 99 29% and RSD(n=6)was 0 57% CONCLUSION:The method is rapid,simple and accurate
